Full strength MS supplemented with 2.0 mg/l NAA and 0.5 mg/l BA was found to be optimal for plant regeneration. The study demonstrated that 2.0 mg/l dicamba and 0.25 mg/l BA induced callus promoted improved frequency compared to zilch shoot regeneration response with other combinations involving 2, 4-D, picloram, KN and BA. Induction of type 2 callus (white and compact) with potential for regeneration was obtained from cold treated (3 days at 10 ☌) immature inflorescence cultured on MS medium containing 2.0 mg/l dicamba and 0.25 mg/l BA. These treatments promoted different callus induction frequencies as well as various callus types such as type 1, type 2 and type 3. The PGRs included auxins: 2, 4-dichlorophenoxy acetic acid (2, 4-D), picloram (Pic), 3, 6-dichloro-2-methoxy benzoic acid (dicamba) and cytokinins: Kinetin (KN), N6-benzyl adenine (BA). ![]() Young inflorescence explants displayed wide-ranging responses for callus induction and plant regeneration when subjected to different cold treatment durations and without cold treatment exposure (control) prior to its inoculation to MS medium supplemented with different concentrations/combinations of plant growth regulators (PGRs). Efficient plant regeneration has been achieved from immature inflorescence derived callus cultures of salt tolerant grass Leptochloa fusca (L.).
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